PRODUCT DESCRIPTION

This sandwich ELISA is designed with polyclonal antibodies coated onto the plate for capturing ANNEXIN A1 (ANXA1) in the tested sample. Following a washing step, captured ANXA1 is tagged with a peroxidase-labelled polyclonal antibody, which binds onto the free epitopes in a dose-dependent manner. After washing away the excess of immunoconjugate, the substrate, 3,3′,5,5′ Tetramethylbenzidine (TMB) with hydrogen peroxide (H2O2) is introduced and a blue color develops, which turns yellow when the reaction is stopped with sulfuric acid. This color is measured at 450nm, and the measured value is directly proportional to the amount of ANXA1 present in the tested sample. Results are expressed in ng/ml, by reference to a calibrated internal reference preparation of human recombinant ANXA1. ANXA1, or Lipocortin I, is a 37 kDa protein from the annexin family with strong anti-inflammatory and pro-resolving activities (Perretti and D’Aquisto, Nat Rev Immunol 2009). ANXA1 is highly expressed in neutrophils, monocytes and macrophages and modestly in mast cells, endothelial cells, epithelial cells and fibroblasts.The 5-ELISA Annexin A1 Antigen kit is a two-site enzyme immunoassay for measuring Annexin A1 antigen in plasma or in any biological fluid where it must be tested.

For measuring Annexin A1 antigen in plasma or in any biological fluid.

PRODUCT CHARACTERISTICS

PRODUCT COMPONENTS

1. COAT: Micro ELISA plate, containing 12×8 well strips, coated with a polyclonal antibody to human ANNEXIN A1, stabilized; the plate is packed in an aluminum pouch sealed in presence of a desiccant.

2. SD: 2 vials of 30 mL Sample Diluent containing aggregated rabbit IgGs, ready to use.

3. WS: 50 mL vial of 20-fold concentrated Wash Solution.

4. CAL: 2 vials of lyophilized Plasma Calibrator, already diluted 1:10 when restored with 2 mL of SD*.

5. C1 (High): 2 vials of lyophilized Control Plasma High, already diluted 1:10when restored with 1 mL of SD* .

6. C2 (Low): 2 vials of lyophilized Control Plasma Low, already diluted 1:10when restored with 1 mL of SD*.

7. IC: 500 µL vial of Anti-(h)-ANNEXIN A1-HRP Conjugate, 50-foldconcentrated.

8. ICD: 25 mL of Conjugate Diluent, ready to use.

9. TMB: 26 mL of Peroxidase Substrate, 3,3′,5,5′-Tetramethylbenzidinecontaining hydrogen peroxide. Ready to use.

10. SA: 7.5 mL vial of 0.50M Sulfuric Acid, ready to use.

*The exact ANNEXIN A1 concentrations are indicated for each lot on the flyer included in the kit.

PRODUCT APPLICATION