PRODUCT DESCRIPTION

Medicago AB Products Are Available Worldwide. For Research Use Only. EC-Blue Enhanced Substrate (TMB) Substrate is a ready-to-use liquid substrate for HRP, specially developed for ELISA applications. TMB Substrate turns deep blue (620-650 nm) in the presence of a peroxidase-labelled conjugate. Color development can be stopped by adding 0.5 M H₂SO₄ (A10-9500-50), which turns the substrate yellow (450 nm). TMB contains a proprietary ready-to-use chromogen substrate mixture. The solution has a straw-yellow color at room temperature and a light greenish tint when stored at 4°C. The color returns to normal when warmed to room temperature. For laboratory use only.

Recommended for ELISA procedures. For Research Use Only. More details here.

PRODUCT CHARACTERISTICS

Ready-to-use liquid substrate.

Substrate for assays using horseradish peroxidase (HRP).

Low background.

High sensitivity.

Long stability.

PRODUCT COMPONENTS

Appearance: Straw yellow color at room temperature. Slightly green tint when stored at +4°C. Color returns to yellow when stored at room temperature.

Protein content: One bottle of EC Blue consist of a solution containing chromogen substrate reagents already mixed, pH 3, 8.

Functional characteristics: Functional testing shows less than 10% average standard deviation from previously approved lots of chromogen. Test performed in the same assay. Background optical densities shows less than 10% deviation from that of previously approved lots when tested in the same assay.

Storage: 2°C to 8°C.

PRODUCT APPLICATION