PRODUCT DESCRIPTION

Medicago AB Products Are Available Worldwide. For Research Use Only. Immobilized Metal ion Affinity Chromatography (IMAC) is a widely used separation method for purifying proteins and peptidesthat have an affinity for metal ions, such as histidine-tagged proteins but also some untagged recombinant or native proteins. Medicago’s IMAC phosphate and elution buffer tablets have been developed exclusively for use in this method. IMAC buffer tablets are supplied in bottles and in blister packs. One tablet of elution buffer yields a 300 mM sodium chloride, 300 mM imidazole, 50 mM sodium phosphate buffer with pH 8.0 at 22°C.

Purification of histidine-tagged and non-histidine-tagged recombinant and native proteins using IMAC chromatography. Available online.

PRODUCT CHARACTERISTICS

Exactly pre-weighed tablets in bottles and blister packs.

Mix elution buffer with phosphate buffer to achieve desired imidazole concentration.

Ultrapure, low UV280 absorbance imidazole.

Formulated from analytical grade chemicals.

Ready to use in minutes.

PRODUCT COMPONENTS

Chemicals: Analytical grade.

Format: Exactly pre-weighed tablets.

Composition: 300 mM Sodium Chloride, 50 mM Sodium Phosphate.

Volume:
Phosphate Buffer: 400 mL.
Elution Buffer: 100 mL

pH: 8.0 ± 0.05 at 22°C.

Shelf Life:
Phosphate Buffer: 5 years after production date.
Elution Buffer: 3 years after production date.

PRODUCT APPLICATION