PRODUCT DESCRIPTION

Medicago AB Products Are Available Worldwide. For Research Use Only. Lens culinaris lectin is isolated from lentil seeds by affinity chromatography. It has 2 subunits and a molecular weight of 46 kDa. The lectin gives two major bands in isoelectrofocusing corresponding to the isomers LCA-A and LCA-B. Lens culinaris is not blood group specific. Its carbohydrate specificity is D-mannose and D-glucose. Adding Mn²⁺ and Ca²⁺ to the reconstitution buffer will enhance hemagglutination activity. LCA contains a hemagglutinin A and B mixture that agglutinates human red blood cells but that is not group-specific. Lens culinaris agglutinates a 2% suspension of human erythrocytes at a lectin concentration of ≤ 6 µg/ml in 0.9% NaCl after 2 hrs at 25°C. Adding 60 mM methylmannoside gives an inhibition with a titer that is at least 16-fold lower than the control. LCA/LcH is supplied as a white lyophilized powder from a buffer containing 1 mM CaCl₂, 1 mM MnCI₂ and 1 mM MgCI₂. No preservatives are added. For laboratory use only.

Use for hemagglutination and cell agglutination studies. Learn more here.

PRODUCT CHARACTERISTICS

Sugar specificity: α-D-mannose and α-D-glucose.

Hemagglutinating activity.

High activity.

PRODUCT COMPONENTS

Appearance: White lyophilized powder or flocculate.

Source: Lentil seeds.

Molecular weight: 46 kDa.

Sugar specificity: α-D-mannose and α-D-glucose.

Activity: Agglutinates human erythrocytes in a 2% blood suspension with lectin concentration ≤ 8 µg/ml in 0.9% NaCl after 2 hrs at 25°C.

Microorganisms: < 100 CFU/g. Protein content: > 85%, OD₂₈₀nm (ε 1.6 = 1 mg/ml).

Identity: SDS-PAGE, three bands – the three isolectins.

Shelf life: > Three years when stored at -20°.

PRODUCT APPLICATION