Medicago AB Products are Available Worldwide. For Research Use Only. TBS Tween 20 is one of the most commonly used biological buffers for plate based immunoassays and Western blotting. It is isotonic, non-toxic to cells and contains a non-ionic detergent. It is used preferably as an antibody diluent and wash reagent in ELISA and Western blotting using alkaline phophatase or peroxidase-conjugated antibodies.