PRODUCT DESCRIPTION

For Research Use Only. Not for Use in Diagnostic Procedures. Two-site ELISA method for the measurement of human PF4 (platelet factor 4), on platelet poor human plasma, or any biological sample where PF4 must be measured. Assay designed with affinity purified rabbit polyclonal antibodies for coating the MicroELISA plate (immunocapture) as well as preparing the Horse Radish Peroxidase (HRP) immunoconjugate marker. This assay measures native or recombinant PF4. It is insensitive to the presence of heparin in the tested sample, and PF4 is measured homogeneously whether its presentation is. The assay calibrator is standardized with the NIBSC International Standard.

For the measurement of Platelet Factor 4 in research applications. For Research Use Only. Not for Use in Diagnostic Procedures.

PRODUCT CHARACTERISTICS

Total Assay Time: 2 hr 15 min

Detection Threshold: < 0.1 ng/mL Dynamic Range: 0.1 to 10 ng/mL Intra-Assay CV: 1 to 5% Inter-Assay CV: 2 to 7% Reference Material established against NIBSC International Standard.

PRODUCT COMPONENTS

Micro ELISA Plate (12 strips of 8 wells)

2x PF4 Sample Diluent

3x PF4 Standard

PF4 Controls (1 x high & 1 x low)

3x Anti-h-PF4-HRP IC

1x Conjugate Diluent

1x Wash Solution

1x TMB

1 x 0.45M Sulfuric Acid

PRODUCT APPLICATION