
PRODUCT DESCRIPTION
Ames MPF 98/100 is a miniaturized, ready to use Ames Test kit in a liquid microplate format according to ISO 11350. It includes all reagents necessary to run 5 samples with strains TA98 and TA100: 5 vials each of semisolid TA98, TA100, plus Booster, Growth -, Exposure -, Reversion Indicator Media, Ampicillin, Positive Controls, lyophilized Post-Mitochondrial Supernatant S9 from Phenobarbital/Naphtoflavone induced rat liver. Not included in the kit are plastic ware and S9 Cofactor solutions. The kits are quality controlled and do not need a separate quality control (phenotypes) like the agar plate Assay in petri dishes when used as an inhouse test.
PRODUCT CHARACTERISTICS
Quality controlled products: strains, rat liver S9, positive controls, ampicillin and media
Certificate of Analysis: Provided with the kit
In accordance with ISO 11350
Excel Calculation Sheet to support Data Interpretation: Free of charge
Comparison with agar plate test: Corresponds to 2 x 240 petri dishes.
Enough Material to process strains individually
Shipped at room temperature from external storage in Germany or from stock in Switzerland
Shipped at room temperature from external storage in Germany or from stock in Switzerland
High sensitivity (LoD)
Excellent correlation with agar plate test (see database and publications on this homepage)
Less hands-on-time and simultaneous processing of several replicates
Automation available – High throughput format
3Rs – 12 fold less consumption of rat liver S9, less test animals
Less error prone due to easy colorimetric read-out
Waste – Significant less plastic waste and thus reduced contaminated waste in environment
PRODUCT COMPONENTS
5 Sample Kit when tested in 2 tester strains TA98 and TA100, +/- S9, 3 Replicates, 6 Doses, Positive & Negative Controls: Strains, Media, Ampicillin, Booster, PB-NF induced rat liver S9, Controls and Excel calculation sheet. S9 Cofactor solutions and plastic ware are not included in the kit.
PRODUCT APPLICATION
Ames mutagenicity assays are important in research applications for:
• the safety of cosmetics and pharmaceuticals
• the exclusion of genotoxic activity in chemicals or pesticides
• the exclusion of mutagenicity in medical devices
• the exclusion of micro-pollutants in drinking water
• the control of absence of genotoxic compounds in surface or waste water, air, soil or sediments
• the research in the field of food ingredients, food packaging
MSDS Documents
IFU Documents